California fish and game (20504820842)

Title: California fish and game
Identifier: californiafishga66_3cali (find matches)
Year: [1] (s)
Authors: California. Dept. of Fish and Game; California. Fish and Game Commission; California. Division of Fish and Game
Subjects: Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons
Publisher: (San Francisco, etc. ) State of California, Resources Agency, Dept. of Fish and Game
Contributing Library: California Department of Fish and Game
Digitizing Sponsor: Internet Archive



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FLORIDA LARCEMOUTH BASS ELECTROPHORETIC STUDIES 137 through starch gel differing distances in response to an electric current to pro- duce a three-banded pattern. The Florida bass pattern is similarly produced by an A gene locus and a B gene locus, each coding for the production of a different subunit (A and B„, respectively). When northern and Florida bass are crossed the genotype AA B„B. translates subunits that randomly combine to form six dimers (AA, AB„ B,B., AB„, B„B„ and B„B„). This appears as a five-banded pattern, hov^ever, since two dimers (B,B, and ABn,) migrate the same distance in starch gel. The F, hybrid shows all bands found in Florida and northern bass plus an additional band (B„BJ. For discussion of MDH phenotypes, MM is used for Florida bass, SS for northern bass, and MS for the hybrid. ( NORTHERN I UARGEMOUTH BASS FLORIDA LARGEMOUTH BASS F, HYBRID
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FIGURE 2. Malate dehydrogenase and tetrazolium oxidase phenotypes of northern and Florida largemouth bass and their F, hybrid. ' Results from study waters showed all MS fish by MDH pattern to show only MM or MS by TO. The frequency of alleles in a given population can be measured by direct counts from the electrophoretic expression of a representative sample (Utter and Allendorf 1977). In this study, the number of M alleles (coding for the B„B„ band) and S alleles (coding for the 83, band) in a population was determined (see Figure 3 for example). The number of MDhI genes in a particular collection is twice the number of individuals sampled because a complementary pair of MDH genes are coded in each individual. A homozygous individual codes in duplicate for a given allelic form and the heterozygous individual codes for two different allelic forms. Childers (pers. commun.) used an additional enzyme system, tetrazolium oxidase (TO), which permitted partial separation of Florida and northern bass (Figure 2). He found that northern bass were homozygous, showing a single